Only two things: the insects themselves, or their physical signs. CDC and EPA state it directly — an infestation is confirmed "only by detecting the pests themselves or their signs, which include fecal spots, blood spots, egg cases, and shed skins." A bite mark is not on that list.
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What are the physical signs, specifically?
Four sourced signs, in the order an inspection usually finds them.
What to look for
4- Rusty-colored blood spots from crushed fecesthe most commonly cited visual sign
- Shed skins (exuviae)bed bugs molt through five nymphal stages before adulthood
- Egg cases, roughly 1/20 inch, white, usually tucked into seams and cracks
- Live insectsadults about 1/5 inch, rusty red to mahogany, wingless
CDC & EPA Joint Statement, 2010.
Beyond sight
1- A sweet, musty odor is a documented sign, alongside the visual evidence
CDC & EPA Joint Statement, 2010.
Where should an inspection start?
At the bed. About 85% of bed bugs turn up in or near it — the mattress, box spring, bed frame and headboard — with the remaining 15% in nearby upholstered furniture, cabinets, baseboards, under wallpaper, and in carpets. They exploit crevices as small as the width of a credit card, and common hiding places sit within about 6 to 8 feet of where someone sleeps, though CDC and EPA note they can travel over 100 feet in a single night.
Interceptor monitors placed under bed legs caught six times more bed bugs than visual searching alone in the underlying research, which is why a thorough inspection uses more than eyesight.
Does a bite confirm it?
No, and this is the point the sourced guidance is most direct about: "the presence of raised wheals, blisters, rashes, or any other dermal symptom associated with arthropod bites should never be used for diagnosis," since several household pests bite and reactions vary widely between people. Marks can also take up to 14 days to appear, and some people show no reaction at all — so an absence of bites is not evidence of absence, and bites alone are not evidence of presence.

Sources
3 cited- What confirms an infestation, and where they concentrate: CDC & EPA, Joint Statement on Bed Bug Control in the United States, 2010 (checked 2026-08-18).
- Where 85% are found and interceptor research: UC IPM Pest Notes Publication 7454, Bed Bugs, May 2013 (checked 2026-08-18).
- Why a bite cannot diagnose an infestation: UC IPM Pest Notes 7454 (checked 2026-08-18).
